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Using crop rotation is beneficial primarily because it enhances nitrogen fixation, particularly when leguminous crops are included in the rotation. Legumes have a symbiotic relationship with nitrogen-fixing bacteria in their root nodules. As these crops are grown and subsequently turned under or left to decay, they enrich the soil with nitrogen, an essential nutrient for plant growth. This natural fertilization process can lead to improved soil health and help sustain crop yields over time.

Incorporating a variety of crops in the rotation also disrupts pest and disease cycles, which can reduce the incidence of crop-specific pests and pathogens. This further contributes to sustainable farming practices. While the incorporation of certain crops can negatively impact nitrogen levels if not managed properly, the overall benefits of using legumes in crop rotation far outweigh these risks and lead to a healthier agro-ecosystem.